Asian Journal of Bio Science

V. D. Deshmukh ¹, H. B. Patil ², J. D. Deshmukh ³, I. A. Madrap ⁴

Affiliations
1 MGM College of Agricultural Biotechnology, Aurangabad (M.S.), IN
2 College of Agriculture Biotechnology, Latur (M.S.), IN
3 Banana Research Station, Nanded (M.S.), IN
4 Department of Botany, Marathwada Agricultural University, Parbhani (M.S.), IN

Abstract

The present study was carried out with emphasis on ISSR profiling and genetic relationship studies on pearl millet using ISSR marker. The experimental material consisted of 15 genotypes of pearl millet. The seeds of 15 genotypes of pearl millet were grown in petriplates and the young leaves from them were used for DNA extraction. Extraction of total genomic DNA was carried out following the methods suggested by Saghai-Maroof et al. (1984) with minor modification. The selected primers generated 123 ISSR bands and the size of the amplification products ranged from 150-35000 bp. The number of bands generated per primer ranged from 09 (CA)₆RG to 19 (AC)₈YA with a mean of 13.6 bands per primer. Out of the bands generated 80 were polymorphic and 43 were monomorphic. The genotype GHB 235 showed maximum similarity (0.92) whereas, minimum was for ICMV 155 (0.55) among the genotypes studied.

Keywords

Pearl Millet, Genetic Diversity, ISSR.

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R. L. Chavhan ¹, V. Y. Patade ², H. B. Patil ³

Affiliations
1 Central Institute for Cotton Research, Post Bag No. 2, Shankarnagar P.O., Nagpur (M.S.), IN
2 Green House Laboratory, Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, BARC, Trombay, Mumbai (M.S.), IN
3 Department of Agricultural Biotechnology, Marathwada Agricultural University, Parbhani (M.S.), IN

Abstract

Callus induction and regeneration in safflower (Carthamus tinctorius L.) cv. Sharda and PBN-12 was optimized using various explants such as hypocotyls, cotyledonary leaves ischolar_mains and apical shoot buds. The cotyledonary leaves exhibited better response for callus induction as compare to ischolar_mains and hypocotyls on MS supplemented with 5mgl^-1 NAA, 0.25mg^-1 BAP and 1% sucrose.

Keywords

Callus Induction, Plantlet Regeneration, Safflower.

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K. J. Wable ¹, H. B. Patil ¹, S. G. Rajput ¹

Affiliations
1 Department of Agril. Biotechnology, Marathwada, Agriculture University Parbhani - 431 402 (M.S.), IN

Abstract

The importance of loading and treatment with a vitrification solution on the survival of Citrus reticulata embryonic axes cryopreserved using vitrification technique (Derudde et al., 1990) were investigated in this paper. Loading treatment was carried out for 20 min. at 25 0C before chemical dehydration with 100 per cent PVS2 for 20 min. After treatment with PVS2 rapid immersion in liquid nitrogen, rapid rewarming, unloading in a 1.2 M sucrose solution for 20 min. Then the culture on solid medium with 0.3 M sucrose for 1 day and growth recovery for 4 weeks on standard medium, survival of C. reticulata embryonic axes reached 60 per cent following the process.

Keywords

Citrus, Vitrification, Cryopreservation.

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